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"It seems that the existence of a higher coliform population encoding just for the b-galactosidase enzyme leads to higher enumerations of coliforms using the Colilert method (Schets et al. 2001)" explain this extract.

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"It seems that the existence of a higher coliform population encoding just for the b-galactosidase enzyme leads to higher enumerations of coliforms using the Colilert method (Schets et al. 2001)" explain this extract.

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Solution

This extract is discussing the relationship between the population of coliform bacteria that encode for the b-galactosidase enzyme and the enumeration (or counting) of coliforms using the Colilert method.

  1. Coliform bacteria: These are a broad class of bacteria found in our environment, including the feces of man and other warm-blooded animals. The presence of coliform bacteria in water or food is an indicator of possible contamination.

  2. B-galactosidase enzyme: This is an enzyme produced by certain types of bacteria, including coliforms. It is involved in the breakdown of lactose into glucose and galactose.

  3. Colilert method: This is a method used to detect and enumerate coliform bacteria in water samples. It involves adding a substrate that the b-galactosidase enzyme can act on. If coliforms are present, they will produce the enzyme, which will then break down the substrate, leading to a color change.

The extract is suggesting that when there is a higher population of coliform bacteria that produce the b-galactosidase enzyme, the Colilert method will yield higher counts of coliforms. This could be because the more of this enzyme that is present, the more substrate can be broken down, leading to a more noticeable color change and thus a higher count.

The reference to Schets et al. 2001 indicates that this observation was made in a study published by these authors in that year.

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