The effect of an antibiotic on the expression of β-galactosidase was studied in E. coli. Bacteria were cultured in the presence of an established lac inducer, with or without the antibiotic. After lysis, the samples were incubated with a chromogenic β-galactosidase substrate. Finally, the amount of the coloured product formed was detected by photometry. The table below shows the O.D. values of different samples. Which antibiotic was used?

An increase in Beta-galactosidase occurs in E. coli cells in response to an increase in which effector molecule A. Galaktose / Galactose B. Glukose / Glucose C. Laktose / Lactose D. Allolaktose / Allolactose

Which among the following statements is NOT TRUE?*1 pointA. Galacto-oligosaccharides are derived from plants.B. In the experiment, the HMOs blocked the growth of the “good” bacteria.C. The human milk oligosaccharides might replace antibiotics for treating infections in infants and adults.D. The results of the research were presented at a meeting of the American Chemical Society in the fall.

β-lactam antibiotics target which of the following:a) The cytoplasmic membraneb) DNA synthesis machineryc) The cell wall synthesisd) Lipopolysaccharide

In which procedures can restriction endonuclease be used?Question 14Answer1.during agarose gel electrophoresis2.SNP genotyping3.checking whether a DNA construct contains an insert4.testing whether induction of the β-galactosidase gene in E. coli was successful

Which of the following antimicrobial drugs can be inactivated by beta-lactamase?SELECT ALL THAT APPLYClavulanateAugmentinMeropenemAmoxicillinStreptomycinChloramphenicol