During the lab project a modified bacterial plasmid called pCR4-TOPO was used as a vector to clone the PCR product. This cloning vector contains the sequence of a gene at the cloning site used for the selection of bacterial colonies. What does this gene encode for? RNA polymerase DNA transcriptase LacZ (beta-galactosidase) A protein that promotes bacteria growth RNA reverse transcriptase

What is being used to carry the new gene into the bacterial cell?

Which enzyme is used to seal a gene sequence into a cloning vector A.DNA polymerase B. DNA ligase C. Helicase D. Reverse transcriptase

They are used in the process of insertion of genes into plasmid vectors during gene cloning and protein expression experiments. Select one: a. Restriction enzymes b. Terminal Transferase c. DNA polymerase d. Ligase

Which of the following describes plasmids?Synthesise proteins.Controls what enters and exits the cell.Small circles of DNA that are used to share useful genes between bacteria.Contains all the instructions the bacterium needs to function and develop.

Which of these enzymes is used in a PCR? RNA polymerase EcoRI DNA polymerase DNA ligase DNA reverse transcriptase