Which enzyme is used to seal a gene sequence into a cloning vector A.DNA polymerase B. DNA ligase C. Helicase D. Reverse transcriptase
Question
Which enzyme is used to seal a gene sequence into a cloning vector A.DNA polymerase B. DNA ligase C. Helicase D. Reverse transcriptase
Solution
The enzyme used to seal a gene sequence into a cloning vector is DNA ligase. So, the correct answer is B. DNA ligase. This enzyme is responsible for joining DNA fragments together by forming a bond between the phosphate backbone of the DNA molecules. This process is crucial in recombinant DNA technology where a desired gene is inserted into a vector DNA for cloning purposes.
Similar Questions
lace the steps of DNA cloning in order from first (top) to last (bottom).The vector is introduced into a host cell, which reproduces.DNA is pasted into a vector, usually a plasmid.A piece of DNA is cut using restriction enzymes.
They are used in the process of insertion of genes into plasmid vectors during gene cloning and protein expression experiments. Select one: a. Restriction enzymes b. Terminal Transferase c. DNA polymerase d. Ligase
Which one is the key step in the cloning of a DNA molecule? Cleaning the DNA from RNA and proteins Ligation of the DNA molecule into a plasmid or cloning vector Digesting the proteins bound to the DNA molecule Extension of the 3'end of the DNA molecules Cleaving the DNA molecule in half
Which of these enzymes is used in a PCR? RNA polymerase EcoRI DNA polymerase DNA ligase DNA reverse transcriptase
An enzyme that catalyzes the cutting and resealing of DNA, and is translated from insertion sequences, isGroup of answer choicesRNA polymerase.DNA ligase.DNA helicase.transposase.DNA polymerase.
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