What can be seen in this picture?Question 3AnswerA.the column used in affinity chromatographyB.the bilayer prepared during in vitro translationC.agarose gel prepared for gel electrophoresisD.cuvette with sample, to measure the protein concentrationE.sample prepared for analysis by the Biuret method

Describe how you would perform a chromatography experiment to produce thechromatogram shown in (c). Assume you have been given the mixture of amino acids anda suitable locating agent. You are provided with common laboratory apparatus. (3) mixture of amino acids is placed as a spot onto a (pencil) baselineplaced into a (suitable) solvent /watera locating agent is added to the (finished) chromatogram (to reveal spots)

The photo shows a certain step of the affinity chromatography purification of the 6xHis-GFP expressed in bacteria.Select the correct statement(s). Question 1Answer1.The picture shows the prepared affinity column.2.The picture shows centrifuged samples.3.The eluted protein collected at the bottom of the tubes (the whitish part below the clear solution).4.In the sample treated with IPTG, the bulk of the the 6xHis tagged protein is found in the supernatant.5.The picture shows the step right before the actual affinity chromatography.

A technique for detecting a protein in a mixture after the proteins are separated by gel electrophoresis and transferred to a membrane is called ____________________.Group of answer choicessandwich ELISAwestern blottingindirect ELISAflow cytometry

Which of the following property of a protein is exploited in gel filtration chromatography

Match the stationary phase (Column I) with its corresponding chromatographytechnique (Column II).Column I Column IIP. Protein A 1. Size exclusion chromatographyQ. Sephadex 2. Ion-exchange chromatographyR. Phenylsepharose 3. Affinity chromatographyS. Diethylaminoethyl cellulose 4. Hydrophobic interaction chromatography(A) P-1, Q-4, R-2, S-3(B) P-3, Q-1, R-4, S-2(C) P-3, Q-4, R-2, S-1(D) P-4, Q-1, R-3, S-2