Match the stationary phase (Column I) with its corresponding chromatographytechnique (Column II).Column I Column IIP. Protein A 1. Size exclusion chromatographyQ. Sephadex 2. Ion-exchange chromatographyR. Phenylsepharose 3. Affinity chromatographyS. Diethylaminoethyl cellulose 4. Hydrophobic interaction chromatography(A) P-1, Q-4, R-2, S-3(B) P-3, Q-1, R-4, S-2(C) P-3, Q-4, R-2, S-1(D) P-4, Q-1, R-3, S-2

The correct answer is (B) P-3, Q-1, R-4, S-2.

Here's why:

P. Protein A is used in Affinity chromatography. Affinity chromatography is a method of separating biochemical mixtures based on a highly specific interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand. So, P matches with 3.

Q. Sephadex is used in Size exclusion chromatography. Size exclusion chromatography (SEC) is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. So, Q matches with 1.

R. Phenylsepharose is used in Hydrophobic interaction chromatography. Hydrophobic interaction chromatography (HIC) is a type of partition chromatography that separates molecules based on their interaction with a hydrophobic ligand coupled to a chromatographic matrix. So, R matches with 4.

S. Diethylaminoethyl cellulose is used in Ion-exchange chromatography. Ion-exchange chromatography (IEC) is a process that allows the separation of ions and polar molecules based on their charge. So, S matches with 2.