Describe how you would perform a chromatography experiment to produce thechromatogram shown in (c). Assume you have been given the mixture of amino acids anda suitable locating agent. You are provided with common laboratory apparatus. (3) mixture of amino acids is placed as a spot onto a (pencil) baselineplaced into a (suitable) solvent /watera locating agent is added to the (finished) chromatogram (to reveal spots)
Question
Describe how you would perform a chromatography experiment to produce thechromatogram shown in (c). Assume you have been given the mixture of amino acids anda suitable locating agent. You are provided with common laboratory apparatus. (3) mixture of amino acids is placed as a spot onto a (pencil) baselineplaced into a (suitable) solvent /watera locating agent is added to the (finished) chromatogram (to reveal spots)
Solution
Sure, here's how you would perform a chromatography experiment:
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First, you need to prepare your chromatography paper. Draw a baseline near the bottom of the paper using a pencil. This is where you will apply your sample. It's important to use a pencil because ink may interfere with your results.
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Apply a small spot of the amino acid mixture onto the baseline. Make sure the spot is as small and concentrated as possible for the best results.
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Once your sample is applied, place the chromatography paper into a beaker or other container with a small amount of suitable solvent, such as water. The baseline with the spot should be above the level of the solvent.
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The solvent will begin to move up the paper, carrying the components of the amino acid mixture with it. Different components will move at different rates, separating along the paper.
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Once the solvent has nearly reached the top of the paper, remove the paper from the beaker and allow it to dry.
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After the paper is dry, apply the locating agent. This will react with the amino acids to produce visible spots.
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The final result is a chromatogram - a pattern of spots that can be used to identify the components of the mixture. The specific pattern will depend on the specific amino acids present in the mixture.
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