Which of the following property of a protein is exploited in gel filtration chromatography
Question
Which of the following property of a protein is exploited in gel filtration chromatography
Solution
In gel filtration chromatography, the property of a protein that is exploited is its size or molecular weight.
Here's a step-by-step explanation:
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Gel filtration chromatography, also known as size exclusion chromatography, separates proteins based on their size.
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The chromatography column is packed with gel-like beads that have tiny pores. These pores are of a specific size.
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When a mixture of proteins is passed through the column, smaller proteins enter the pores and take longer to pass through the column.
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Larger proteins, on the other hand, cannot enter the pores and thus travel through the column more quickly.
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In this way, proteins are separated based on their size, with larger proteins eluting from the column before smaller ones.
So, the property of a protein that is exploited in gel filtration chromatography is its size or molecular weight.
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