A protein was run under native conditions in a gel filtration column and was calculated to be of 120 kDa in size. The molecular weight of the protein in SDS-PAGE was found to be 30 kDa. The oligomeric status of the protein is

A protein was eluted at 15 ml when passed through a gel filtration column with a void volume of 5 ml and a pore volume of 1 ml. The Kd value of the protein is

Which of the following property of a protein is exploited in gel filtration chromatography

A sample of protein was run on an SDS-PAGE together with a molecular weight marker ladder. The relative mobilities of the markers and the unknown protein were calculated. The log molecular weights of the markers were plotted against their relative mobilities resulting in the following standard curve. If the relative mobility of the unknown protein was 4.2cm, calculate the molecular weight of the unknown protein.

of an unknown protein are dissolved in enough solvent to make 5.00mL of solution. The osmotic pressure of this solution is measured to be 0.0773atm at 25.0°C.Calculate the molar mass of the protein. Round your answer to 3 significant digits.

Briefly describe what each of the following tells you about the characteristics of the proteins ofinterest described in the statement (Note: The protein is not the same one in each statement):(a) When subjected to dialysis using tubing with a molecular mass cutoff of 3,000 Da, a protein ofinterest is found in the retentate;(b) When subjected to ultrafiltration using a membrane with a molecular mass cutoff of 10,000 Da,a protein of interest is found in the filtrate;(c) When the protein was subjected to ion-exchange chromatography using an anion-exchangecolumn and a buffer of pH 8.0, a protein of interest was bound to the column;(d) When a protein of interest was subjected to isoelectric focusing, the protein migrated to aposition of approximately pH 7.2 in the pH gradient of the gel;(e) When a protein of interest was subjected to SDS-PAGE in both the presence and absence ofmercaptoethanol, the protein appeared as three bands at molecular mass 42,000, 45,000, and48,000 Da;(f) When a solution with various proteins was heated to 60 °C, the protein of interest was found inthe precipitate obtained upon centrifugation of the solution