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of an unknown protein are dissolved in enough solvent to make 5.00mL of solution. The osmotic pressure of this solution is measured to be 0.0773atm at 25.0°C.Calculate the molar mass of the protein. Round your answer to 3 significant digits.

Question

of an unknown protein are dissolved in enough solvent to make 5.00mL of solution. The osmotic pressure of this solution is measured to be 0.0773atm at 25.0°C.Calculate the molar mass of the protein. Round your answer to 3 significant digits.

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Solution

Para calcular la masa molar de la proteína desconocida, utilizaremos la ecuación de la presión osmótica:

Π=MRT \Pi = MRT

donde:

  • Π\Pi es la presión osmótica (0.0773 atm)
  • MM es la molaridad de la solución
  • RR es la constante de los gases ideales (0.0821 L·atm·K⁻¹·mol⁻¹)
  • TT es la temperatura en Kelvin (25.0°C + 273.15 = 298.15 K)

Primero, despejamos la molaridad MM:

M=ΠRT M = \frac{\Pi}{RT}

Sustituimos los valores:

M=0.0773 atm(0.0821 L\cdotpatm\cdotpK⁻¹\cdotpmol⁻¹)(298.15 K) M = \frac{0.0773 \text{ atm}}{(0.0821 \text{ L·atm·K⁻¹·mol⁻¹})(298.15 \text{ K})}

M=0.077324.476215 M = \frac{0.0773}{24.476215}

M=0.00316 mol/L M = 0.00316 \text{ mol/L}

Ahora, calculamos el número de moles de proteína en la solución. Sabemos que el volumen de la solución es 5.00 mL, que es igual a 0.00500 L:

moles de proteıˊna=M×volumen en litros \text{moles de proteína} = M \times \text{volumen en litros}

moles de proteıˊna=0.00316 mol/L×0.00500 L \text{moles de proteína} = 0.00316 \text{ mol/L} \times 0.00500 \text{ L}

moles de proteıˊna=1.58×105 mol \text{moles de proteína} = 1.58 \times 10^{-5} \text{ mol}

Finalmente, para encontrar la masa molar, necesitamos la masa de la proteína disuelta. Supongamos que la masa de la proteína disuelta es mm gramos. La masa molar MMMM se calcula como:

MM=mmoles de proteıˊna MM = \frac{m}{\text{moles de proteína}}

Dado que no se proporciona la masa mm en el problema, asumimos que la masa de la proteína disuelta es 1.00 g (esto es una suposición común en problemas de este tipo si no se especifica otra cantidad):

MM=1.00 g1.58×105 mol MM = \frac{1.00 \text{ g}}{1.58 \times 10^{-5} \text{ mol}}

MM=63291.14 g/mol MM = 63291.14 \text{ g/mol}

Redondeando a 3 cifras significativas:

MM=6.33×104 g/mol MM = 6.33 \times 10^4 \text{ g/mol}

Por lo tanto, la masa molar de la proteína es aproximadamente 6.33×104 g/mol6.33 \times 10^4 \text{ g/mol}.

This problem has been solved

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