of an unknown protein are dissolved in enough solvent to make 5.00mL of solution. The osmotic pressure of this solution is measured to be 0.0773atm at 25.0°C.Calculate the molar mass of the protein. Round your answer to 3 significant digits.
Question
of an unknown protein are dissolved in enough solvent to make 5.00mL of solution. The osmotic pressure of this solution is measured to be 0.0773atm at 25.0°C.Calculate the molar mass of the protein. Round your answer to 3 significant digits.
Solution
Para calcular la masa molar de la proteína desconocida, utilizaremos la ecuación de la presión osmótica:
donde:
- es la presión osmótica (0.0773 atm)
- es la molaridad de la solución
- es la constante de los gases ideales (0.0821 L·atm·K⁻¹·mol⁻¹)
- es la temperatura en Kelvin (25.0°C + 273.15 = 298.15 K)
Primero, despejamos la molaridad :
Sustituimos los valores:
Ahora, calculamos el número de moles de proteína en la solución. Sabemos que el volumen de la solución es 5.00 mL, que es igual a 0.00500 L:
Finalmente, para encontrar la masa molar, necesitamos la masa de la proteína disuelta. Supongamos que la masa de la proteína disuelta es gramos. La masa molar se calcula como:
Dado que no se proporciona la masa en el problema, asumimos que la masa de la proteína disuelta es 1.00 g (esto es una suposición común en problemas de este tipo si no se especifica otra cantidad):
Redondeando a 3 cifras significativas:
Por lo tanto, la masa molar de la proteína es aproximadamente .
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