If an enzyme makes a single cut on a plasmid, how many bands will you see on the gel?fourthreetwoone

A circular plasmid has three different but unique restriction sites for enzymes‘a’, ‘b’ and ‘c.’ When enzymes ‘a’ and ‘b’ are used together, two fragments ofequal size are generated. Enzyme ‘c’ creates fragments of equal size only fromone of the fragments generated by those cleaved by ‘a’ and ‘b’. The plasmid istreated with a mixture of ‘a’, ‘b’ and ‘c’ and analysed by agarose gelelectrophoresis. The number of bands observed in the gel is

A researcher is working with a protein that contains 3 subunits of differing molecular weights. After the researcher performs SDS-PAGE, how many distinct bands should he see on the gel after Coomassie blue staining?ThreeTwoOneFour

The pCR4-TOPO plasmid used in the lab has 3956 bp and contains two EcoRI sites flanking the cloning site. The PCR product of the ACTB gene used for the ligation was ~2000 bp. After transforming and selecting the cells, a liquid LB media was grown form a colony overnight and the plasmid isolated from the cells. Thereafter the plasmid was digested with EcoRI and ran in an agarose gel. What approximate band sizes did you expect to see in the gel? 1 band of ~6000 bp 4 bands each of ~1500 bp 3 bands of ~1000 bp, ~2000 bp, and ~4000 bp 2 bands, one of ~2000 bp and one of ~4000 bp I expect to see no bands on the gel

A single digest meansthe plasmid has been cut with two enzymesthe plasmid has been cut with one enzymethe plasmid has not been cut with any enzymes

5.Which word describes the change in shape of an enzyme?