A circular plasmid has three different but unique restriction sites for enzymes‘a’, ‘b’ and ‘c.’ When enzymes ‘a’ and ‘b’ are used together, two fragments ofequal size are generated. Enzyme ‘c’ creates fragments of equal size only fromone of the fragments generated by those cleaved by ‘a’ and ‘b’. The plasmid istreated with a mixture of ‘a’, ‘b’ and ‘c’ and analysed by agarose gelelectrophoresis. The number of bands observed in the gel is
Question
A circular plasmid has three different but unique restriction sites for enzymes‘a’, ‘b’ and ‘c.’ When enzymes ‘a’ and ‘b’ are used together, two fragments ofequal size are generated. Enzyme ‘c’ creates fragments of equal size only fromone of the fragments generated by those cleaved by ‘a’ and ‘b’. The plasmid istreated with a mixture of ‘a’, ‘b’ and ‘c’ and analysed by agarose gelelectrophoresis. The number of bands observed in the gel is
Solution
The number of bands observed in the gel would be three. Here's why:
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Enzymes 'a' and 'b' are used together and they generate two fragments of equal size. This means that the plasmid is cut into two equal halves.
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Enzyme 'c' then cuts one of these fragments into two equal parts. This means that one of the halves is further divided into two quarters.
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When the plasmid is treated with a mixture of 'a', 'b' and 'c', it is cut into three pieces: one half and two quarters.
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When analyzed by agarose gel electrophoresis, each fragment will migrate to a different position in the gel based on its size, resulting in three distinct bands.
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