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A single digest meansthe plasmid has been cut with two enzymesthe plasmid has been cut with one enzymethe plasmid has not been cut with any enzymes

Question

A single digest meansthe plasmid has been cut with two enzymesthe plasmid has been cut with one enzymethe plasmid has not been cut with any enzymes

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Solution

A single digest means the plasmid has been cut with one enzyme. This process involves using a specific restriction enzyme to cut the DNA at a specific sequence. If two enzymes were used, it would be called a double digest. If the plasmid has not been cut with any enzymes, it remains intact.

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