Which of the following can lead to contamination when streaking an agar plate? Group of answer choices All of the other responses are equally possible Flaming the loop immediately after picking off a colony Forgetting to flame the loop before picking off a colony Re-crossing the first set of streaks Streaking with the side of a loop Forgetting to flame the loop between streaks
Question
Which of the following can lead to contamination when streaking an agar plate?
Group of answer choices
All of the other responses are equally possible
Flaming the loop immediately after picking off a colony
Forgetting to flame the loop before picking off a colony
Re-crossing the first set of streaks
Streaking with the side of a loop
Forgetting to flame the loop between streaks
Solution
All of the other responses are equally possible.
Each of the options listed can potentially lead to contamination when streaking an agar plate.
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Flaming the loop immediately after picking off a colony: This can potentially kill the bacteria you just picked up, leading to a failed streak plate.
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Forgetting to flame the loop before picking off a colony: This can introduce unwanted bacteria or other microorganisms to your plate, leading to contamination.
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Re-crossing the first set of streaks: This can spread bacteria from the first set of streaks to other parts of the plate, leading to a non-isolated colony.
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Streaking with the side of a loop: This can potentially scrape off the agar from the plate, leading to a failed streak plate.
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Forgetting to flame the loop between streaks: This can spread bacteria from one streak to another, leading to a non-isolated colony.
Therefore, all of these actions can potentially lead to contamination when streaking an agar plate.
Similar Questions
Select ALL of the following statements that are INCORRECT with regards to the streaking technique: Group of answer choices The final set of streaks must always finish by touching the first set of streaks. The objective of streaking out a bacterial sample is to increase the bacterial numbers on the surface of the agar plate so that a loan of bacteria is formed. In order to dilute the bacteria, each set of streaks must begin within the previous set. The purpose of flaming the loop between each set of streaks is to reduce the bacterial numbers transferred to the subsequent set of streaks.
A laboratory worker prepares a streak plate of a bacterial culture. While there is substantial growth on the plate, there is very poor separation of isolated colonies. All of the following are possible reasons for the poor separation except: Group of answer choices Streaking with the side of a loop Failure to flame the loop between streaks Insufficient inoculum on the loop Re-crossing over the first set of streaks
In the viable plate count method, a measured sample of a culture is evenly spread across an agar surface and incubated. Each _____ represents one _____ from the sample. A. cell, cell B. cell, colony C. colony, cell D. species, colony E. generation, cell Question 29 options:
Imagine that you forgot to flame the loop before streaking the inoculum from the first quadrant into the second quadrant. What is the most likely consequence of this error?Too much bacterial growth outside the first quadrant.Contamination of the broth culture.Too little bacterial growth outside the first quadrant.Contamination of the Petri plate culture.
Which of the following is a description of the elevation of a bacterial colony above the surface of an agar plate? Group of answer choices Filamentous Irregular Round Convex Rhizoid
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