Select ALL of the following statements that are INCORRECT with regards to the streaking technique: Group of answer choices The final set of streaks must always finish by touching the first set of streaks. The objective of streaking out a bacterial sample is to increase the bacterial numbers on the surface of the agar plate so that a loan of bacteria is formed. In order to dilute the bacteria, each set of streaks must begin within the previous set. The purpose of flaming the loop between each set of streaks is to reduce the bacterial numbers transferred to the subsequent set of streaks.

Which of the following can lead to contamination when streaking an agar plate? Group of answer choices All of the other responses are equally possible Flaming the loop immediately after picking off a colony Forgetting to flame the loop before picking off a colony Re-crossing the first set of streaks Streaking with the side of a loop Forgetting to flame the loop between streaks

A laboratory worker prepares a streak plate of a bacterial culture. While there is substantial growth on the plate, there is very poor separation of isolated colonies. All of the following are possible reasons for the poor separation except: Group of answer choices Streaking with the side of a loop Failure to flame the loop between streaks Insufficient inoculum on the loop Re-crossing over the first set of streaks

A sterile agar plate, I, is streaked with a pure culture of bacteria by means of aseptic techniques. Paper discs treated with the antibiotics Aureomycin (A) and penicillin (P) are placed at opposite sides of the plate, as shown in the diagram above. The plate is examined after a 24-hour incubation period, and a clear ring is discovered around disc A, but not around disc P. Within the clear ring around disc A, a single bacterial colony with physical characteristics like those of the pure culture is observed. A second sterile agar plate, II, is then streaked with this single colony and also incubated with antibiotics.

In the viable plate count method, a measured sample of a culture is evenly spread across an agar surface and incubated. Each _____ represents one _____ from the sample. A. cell, cell B. cell, colony C. colony, cell D. species, colony E. generation, cell Question 29 options:

In the enumeration of bacteria in a culture by a plate count, all of the following apply when determining the numbers except: Group of answer choices All bacteria in the culture are counted Statistically only plates with between 30-300 colonies should be counted It is assumed that each colony arose from division of one or a few cells All of the answer choices are correct The medium must contain nutrients used by the bacteria