In the viable plate count method, a measured sample of a culture is evenly spread across an agar surface and incubated. Each _____ represents one _____ from the sample. A. cell, cell B. cell, colony C. colony, cell D. species, colony E. generation, cell Question 29 options:

In the enumeration of bacteria in a culture by a plate count, all of the following apply when determining the numbers except: Group of answer choices All bacteria in the culture are counted Statistically only plates with between 30-300 colonies should be counted It is assumed that each colony arose from division of one or a few cells All of the answer choices are correct The medium must contain nutrients used by the bacteria

Besides being used for plate counts, spread plates in combination with serial dilutions can be used as a method for isolating pure cultures. Group of answer choices True False

Plate Count Method

Which of these methods can be used to obtain a total cell estimate? Group of answer choices Most probably number estimations Any of the methods listed Plate counts of serial dilutions Optical density measurement

Which of the following techniques is the best way to directly count viable cells?ANSWERMeasuring the turbidity of a solutionPlate countsMeasuring the consumption of oxygenDirect microscopic counting of only motile cells