Imagine that you forgot to flame the loop before streaking the inoculum from the first quadrant into the second quadrant. What is the most likely consequence of this error?Too much bacterial growth outside the first quadrant.Contamination of the broth culture.Too little bacterial growth outside the first quadrant.Contamination of the Petri plate culture.

A laboratory worker prepares a streak plate of a bacterial culture. While there is substantial growth on the plate, there is very poor separation of isolated colonies. All of the following are possible reasons for the poor separation except: Group of answer choices Streaking with the side of a loop Failure to flame the loop between streaks Insufficient inoculum on the loop Re-crossing over the first set of streaks

How should the inoculating loop be sterilised before using it to transfer bacteria to the Petri dish?Place it in the autoclaveDip it in the agar jellyHold it in the Bunsen flamePlace it in the nutrient broth

Which of the following can lead to contamination when streaking an agar plate? Group of answer choices All of the other responses are equally possible Flaming the loop immediately after picking off a colony Forgetting to flame the loop before picking off a colony Re-crossing the first set of streaks Streaking with the side of a loop Forgetting to flame the loop between streaks

During asceptic transfer, the innoculation loop should be flamed after every contact with the bacterial culture.

Select ALL of the following statements that are INCORRECT regarding microbiology lab procedures Group of answer choices For safety’s sake the Bunsen burner should be left sitting underneath the gas taps or gas tubing. To sterilise a loop you need to hold it in the bunsen burner until it glows red hot. Always drain the ethanol off the spreader or forcepts before flaming to avoid dripping burning ethanol on anything. Set up your work area in a manner that enables you to safely use the space without reaching across the Bunsen burner. When flaming the spreader or the forceps, hold the spreader tilted down so that any flaming ethanol runs away from your hands. It is safe to hold any flaming instrument over the ethanol beaker.