During asceptic transfer, the innoculation loop should be flamed after every contact with the bacterial culture.
Question
During asceptic transfer, the innoculation loop should be flamed after every contact with the bacterial culture.
Solution
Yes, that's correct. During aseptic transfer, the inoculation loop should be flamed after every contact with the bacterial culture. This is done to kill any remaining bacteria on the loop and prevent contamination. The steps are as follows:
- Flame the loop until it is red hot to sterilize it.
- Allow the loop to cool.
- Dip the loop into the bacterial culture to pick up bacteria.
- Flame the loop again to kill the bacteria.
- Transfer the bacteria to the new medium.
- Flame the loop once more to kill any remaining bacteria.
Remember, the goal of aseptic technique is to prevent contamination of the sample, the environment, and the person performing the procedure.
Similar Questions
After inoculating and incubating an agar slant from a culture of E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Check all that apply.) Select 3 correct answer(s) Question 6 options: There is no growth on the slant. There are red, yellow, and white colonies on the slant. There is fungal growth in the original E. coli culture tube. There are colonies of similar morphology on the slant.
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