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During asceptic transfer, the innoculation loop should be flamed after every contact with the bacterial culture.

Question

During asceptic transfer, the innoculation loop should be flamed after every contact with the bacterial culture.

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Solution

Yes, that's correct. During aseptic transfer, the inoculation loop should be flamed after every contact with the bacterial culture. This is done to kill any remaining bacteria on the loop and prevent contamination. The steps are as follows:

  1. Flame the loop until it is red hot to sterilize it.
  2. Allow the loop to cool.
  3. Dip the loop into the bacterial culture to pick up bacteria.
  4. Flame the loop again to kill the bacteria.
  5. Transfer the bacteria to the new medium.
  6. Flame the loop once more to kill any remaining bacteria.

Remember, the goal of aseptic technique is to prevent contamination of the sample, the environment, and the person performing the procedure.

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