After inoculating and incubating an agar slant from a culture of E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Check all that apply.) Select 3 correct answer(s) Question 6 options: There is no growth on the slant. There are red, yellow, and white colonies on the slant. There is fungal growth in the original E. coli culture tube. There are colonies of similar morphology on the slant.

When investigating the effect of antimicrobials on the growth of bacteria, it is important that the antimicrobials are only tested on one type of bacteria. Therefore, it is essential that aseptic technique is used when carrying out this required practical.Below are methods of aseptic technique that should be used when carrying out this practical.Match each method to the reason why it is carried out.Do not place tape all the way around the lid of the agar plate.Secure the lid of the agar plate with tape.Incubate the agar plate at 25 °C for 48 hours.Keep the agar plate and other equipment close to the Bunsen burner.Possible AnswersTo prevent the lid from coming off and the culture from becoming contaminated with airborne microorganisms.To prevent the lid from coming off and the bacteria from contaminating the air.To allow oxygen in, preventing the growth of dangerous, anaerobic bacteria.To ensure they are close to the updraft created by the flame, which will limit contamination by airborne microorganisms and dust particles.To allow oxygen out, preventing the growth of dangerous, anaerobic bacteria.To prevent the growth of human pathogens which thrive at body temperature.To ensure they are close to the updraft created by the flame, which will make it easier when dipping the discs in the antiseptic.I DON'T KNOWRESETSUBMIT ANSWER

Resistant and nonresistant E. coli bacteria samples were spread on the agar plates below and incubated for 24 hours.  The streptomycin negative with resistant E. coli. appears spread throughout the plate.The streptomycin positive with resistant E. coli. appears spread throughout the plate.The streptomycin negative with non-resistant E. coli. appears spread throughout the plate.The streptomycin positive with non-resistant E. coli. appears concentrated at three points on the plate. Select the true statements from the list below.Check All That ApplyThe resistant E. coli strain grows on streptomycin negative plates.The resistant E. coli strain grows on streptomycin negative plates.The nonresistant E. coli strain grows on streptomycin negative plates.The nonresistant E. coli strain grows on streptomycin negative plates.The nonresistant E. coli strain grows the same on streptomycin positive or negative plates.The nonresistant E. coli strain grows the same on streptomycin positive or negative plates.The resistant E. coli strain grows on streptomycin positive plates.The resistant E. coli strain grows on streptomycin positive plates.Both resistant and nonresistant E. coli strain show no growth on streptomycin positive plates.

During asceptic transfer, the innoculation loop should be flamed after every contact with the bacterial culture.

Which of the following is a description of the elevation of a bacterial colony above the surface of an agar plate? Group of answer choices Filamentous Irregular Round Convex Rhizoid

You inoculated a pure culture of unknown bacteria into a tube containing thioglycollate medium to study their metabolic relationship with oxygen. The culture was incubated for an appropriate length of time at the optimal growth temperature of the organism. When you examined the culture, you observed that although the organism grew throughout the medium, the growth was denser at the surface. What would you conclude about the oxygen requirements for this unknown species?ANSWERThe unknown is an obligate anaerobe.The unknown is an obligate aerobe.The unknown is a microaerophile.The unknown is a facultative anaerobe.