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You inoculated a pure culture of unknown bacteria into a tube containing thioglycollate medium to study their metabolic relationship with oxygen. The culture was incubated for an appropriate length of time at the optimal growth temperature of the organism. When you examined the culture, you observed that although the organism grew throughout the medium, the growth was denser at the surface. What would you conclude about the oxygen requirements for this unknown species?ANSWERThe unknown is an obligate anaerobe.The unknown is an obligate aerobe.The unknown is a microaerophile.The unknown is a facultative anaerobe.

Question

You inoculated a pure culture of unknown bacteria into a tube containing thioglycollate medium to study their metabolic relationship with oxygen. The culture was incubated for an appropriate length of time at the optimal growth temperature of the organism. When you examined the culture, you observed that although the organism grew throughout the medium, the growth was denser at the surface. What would you conclude about the oxygen requirements for this unknown species?ANSWERThe unknown is an obligate anaerobe.The unknown is an obligate aerobe.The unknown is a microaerophile.The unknown is a facultative anaerobe.

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Solution

The unknown is a facultative anaerobe.

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