nucleotide is added to primers in pcr cycle is it added to 5' 3' or 3' 5'

A scientist designs a primer, which is a short oligonucleotide, for use in a DNA sequencing reaction. The primer is complementary to the DNA template shown below.3’-AGCTAGCGATCGGACGAT-5’Which of the following shows the sequence and orientation of the primer?Choose 1 answer:Choose 1 answer:(Choice A)   3’-TCGATCGCTAGCCTGCTA-5’A3’-TCGATCGCTAGCCTGCTA-5’(Choice B)   5’-TCGATCGCTAGCCTGCTA-3’B5’-TCGATCGCTAGCCTGCTA-3’(Choice C)   5’-CTAGCTATCGATTCATCG-3’C5’-CTAGCTATCGATTCATCG-3’(Choice D)   3’-CTAGCTATCGATTCATCG-5’D3’-CTAGCTATCGATTCATCG-5’

Organize the following steps of a PCR cycle in the correct sequence.The mixture is cooled to allow the binding of primers to the end of target DNA sequences.Free nucleotides are added in the 5’ to 3’ direction by the Taq DNA polymerase.The reaction mixture is heated to separate the double-stranded DNA.Group of answer choices1, 2, 31, 3, 22, 1, 32, 3, 13, 1, 23, 2, 1

Why are the two strands of nucleotides in a DNA molcule required to be separated during PCR?

Which of the following correctly explains why primers are used in PCR? A. DNA replication requires a free 3′ hydroxyl group. B. DNA replication requires a free 5′ phosphate group. C. DNA replication requires a free 5′ hydroxyl group. D. DNA replication requires a free 3′ phosphate group.

Why does an RNA primer need to be added to the DNA template for DNA replication?Multiple choice question.RNA polymerase needs to be primed to release each template strand of DNA.DNA polymerase can only add nucleotides to an existing strand.DNA polymerase cannot join existing strands of DNA together.Each DNA double helix must be unwound before replication can begin.