Organize the following steps of a PCR cycle in the correct sequence.The mixture is cooled to allow the binding of primers to the end of target DNA sequences.Free nucleotides are added in the 5’ to 3’ direction by the Taq DNA polymerase.The reaction mixture is heated to separate the double-stranded DNA.Group of answer choices1, 2, 31, 3, 22, 1, 32, 3, 13, 1, 23, 2, 1

The PCR reaction is based on A. DNA replication B. Temperature cycles C. Copies of a target DNA sequence D. All the options

Which of the following correctly matches the step of polymerase chain reaction (PCR) with its events?Choose 1 answer:Choose 1 answer:(Choice A)   Extension - reaction is cooled and primers bind to complementary sequences on template DNA.AExtension - reaction is cooled and primers bind to complementary sequences on template DNA.(Choice B)   Annealing - Taq polymerase extends the primers, synthesizing DNABAnnealing - Taq polymerase extends the primers, synthesizing DNA(Choice C, Checked)   Denaturation - high temperature separates the DNACDenaturation - high temperature separates the DNA

1. QuestionPolymerase chain reaction (PCR) is a DNA manipulation technique used to: Cut DNA molecules at specific sequences of nucleotides Separate DNA molecules according to size Join molecules of DNA together Copy specific regions of DNA2. QuestionWhat occurs in the first step of PCR? DNA strands are separated by heating them Primers bind to target DNA strands Taq DNA polymerase builds a new DNA molecule using the target strand as a template3. QuestionWhat occurs in the second step of PCR? DNA strands are separated by heating them Primers bind to target DNA strands Taq DNA polymerase builds a new DNA molecule using the target strand as a template4. QuestionWhat occurs in the final step of PCR? DNA strands are separated by heating them Primers bind to target DNA strands Taq DNA polymerase builds a new DNA molecule using the target strand as a template5. QuestionWhy are the two strands of nucleotides in a DNA molcule required to be separated during PCR?6. QuestionReferring to the image below, which primer will bind to the sequence of nucleotides? Primer 1 Primer 2 Neither primer will bind7. QuestionHow does Taq DNA polymerase extend a new strand of nucleotides? By adding nucleotides that are identical to the original strand By adding nucleotides that are complementary to the original strand By joining primers together

Which single statement regarding the Polymerase Chain Reaction (PCR) is FALSE?Group of answer choicesPCR amplifies a specific DNA fragmentBoth primers are complimentary to the same short sequenceA heat-stable DNA polymerase is required to synthesize new DNAHeat (95ºC) is required to separate the DNA strands

nucleotide is added to primers in pcr cycle is it added to 5' 3' or 3' 5'