A scientist designs a primer, which is a short oligonucleotide, for use in a DNA sequencing reaction. The primer is complementary to the DNA template shown below.3’-AGCTAGCGATCGGACGAT-5’Which of the following shows the sequence and orientation of the primer?Choose 1 answer:Choose 1 answer:(Choice A)   3’-TCGATCGCTAGCCTGCTA-5’A3’-TCGATCGCTAGCCTGCTA-5’(Choice B)   5’-TCGATCGCTAGCCTGCTA-3’B5’-TCGATCGCTAGCCTGCTA-3’(Choice C)   5’-CTAGCTATCGATTCATCG-3’C5’-CTAGCTATCGATTCATCG-3’(Choice D)   3’-CTAGCTATCGATTCATCG-5’D3’-CTAGCTATCGATTCATCG-5’

nucleotide is added to primers in pcr cycle is it added to 5' 3' or 3' 5'

Below is the position of primer binding sites on a sequence of DNA that is to be amplified using PCR. Which pair of primers will amplify this sequence?5’ GCATCAATCT …. AAGCTACCGA 3’Group of answer choices5’ AGATTGATGC 3’ and 5’ AAGCTACCGA 3’5’ GCATCAATCT 3' and 5' AAGCTACCGA 3’5’ AGATTGATGC 3’ and 5’ TCGGTAGCTT 3’5’ GCATCAATCT 3’ and 5’ TCGGTAGCTT 3’

The polymerase chain reaction needs the following A. Two primers must flank the sequence of interest on opposite strands B. One primer on the template strand C. Two primers on the same strand D. The two primers must be orientated with 5' ends facing each other

A short polynucleotide with a free 3’ end, bound by complementary base pairing to the template strand and elongated with DNA nucleotides during DNA replication.Group of answer choicesRNA primertelomereOkazaki fragmentpromoterDNA primerNext

Organize the following steps of a PCR cycle in the correct sequence.The mixture is cooled to allow the binding of primers to the end of target DNA sequences.Free nucleotides are added in the 5’ to 3’ direction by the Taq DNA polymerase.The reaction mixture is heated to separate the double-stranded DNA.Group of answer choices1, 2, 31, 3, 22, 1, 32, 3, 13, 1, 23, 2, 1