A globular protein was incubated separately in 1 M, 2M, 5M and 8M urea for 10 hours at 370C. Which of the protein fraction will have the least distribution coefficient (Kd) value? (a)1 M (b)2 M (c)4 M (d)8 M
Question
A globular protein was incubated separately in 1 M, 2M, 5M and 8M urea for 10 hours at 370C. Which of the protein fraction will have the least distribution coefficient (Kd) value? (a)1 M (b)2 M (c)4 M (d)8 M
Solution
The distribution coefficient (Kd) is a measure of how a protein distributes itself between two immiscible phases. In the context of protein denaturation with urea, a higher concentration of urea will lead to a greater degree of protein unfolding or denaturation.
This is because urea is a chaotropic agent, which disrupts the hydrophobic interactions that help to stabilize the native structure of the protein. As the concentration of urea increases, more of these interactions are disrupted, leading to a greater degree of unfolding.
Therefore, the protein fraction that is incubated in 8M urea will have the least distribution coefficient (Kd) value because it will be the most denatured. So, the answer is (d) 8 M.
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