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1 pointThe distribution Coefficient (Kd) value of X, Y and Z are 1, 5 and 10 respectively. A mixture of an equal amount of X, Y and Z was injected to a column. The order of their elution is

Question

1 pointThe distribution Coefficient (Kd) value of X, Y and Z are 1, 5 and 10 respectively. A mixture of an equal amount of X, Y and Z was injected to a column. The order of their elution is

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Solution

The order of elution will be based on the distribution coefficient (Kd) values. The lower the Kd value, the faster the compound will elute from the column.

So, in this case, the order of elution will be:

  1. X (Kd = 1)
  2. Y (Kd = 5)
  3. Z (Kd = 10)

This is because X has the lowest Kd value, so it will elute first. Y has the next lowest Kd value, so it will elute second. Z has the highest Kd value, so it will elute last.

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