Your solution is 10000 times more dilute than the original bacterial sample (ie 1x10-4). Your take 0.5ml of this solution and add it to 4.5ml saline. Once mixed, 0.5ml of that diluted cell suspension is placed in another tube with 4.5 ml of sterile saline. How many times is the original cell suspension diluted now? (Please type your numerical answer eg 1 or 10 or 100 or 1000 etc)
Question
Your solution is 10000 times more dilute than the original bacterial sample (ie 1x10-4). Your take 0.5ml of this solution and add it to 4.5ml saline. Once mixed, 0.5ml of that diluted cell suspension is placed in another tube with 4.5 ml of sterile saline. How many times is the original cell suspension diluted now? (Please type your numerical answer eg 1 or 10 or 100 or 1000 etc)
Solution
The original cell suspension was diluted 10000 times (1x10^-4).
Step 1: When you take 0.5ml of this solution and add it to 4.5ml saline, you are diluting it by a factor of 10 (5ml total / 0.5ml of the solution).
Step 2: Then, when you take 0.5ml of that diluted cell suspension and place it in another tube with 4.5 ml of sterile saline, you are again diluting it by a factor of 10 (5ml total / 0.5ml of the solution).
So, the total dilution factor is the product of the dilution factors at each step.
Total dilution = Original dilution x Step 1 dilution x Step 2 dilution Total dilution = 10000 x 10 x 10 = 1,000,000 times.
So, the original cell suspension is now diluted 1,000,000 times.
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