Which of the following uses a known fluorescent antibody to detect the presence of a target antigen?Multiple choice question.Direct antigen testRadioimmunoassayIndirect immunofluorescence testDirect immunofluorescence testDirect ELISACapture ELISA
Question
Which of the following uses a known fluorescent antibody to detect the presence of a target antigen?Multiple choice question.Direct antigen testRadioimmunoassayIndirect immunofluorescence testDirect immunofluorescence testDirect ELISACapture ELISA
Solution
The method that uses a known fluorescent antibody to detect the presence of a target antigen is the Direct immunofluorescence test.
Here's a step-by-step explanation:
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In a Direct immunofluorescence test, the test sample (which could be a tissue sample, blood sample, etc.) is first collected.
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This sample is then exposed to a specific antibody that is tagged with a fluorescent dye. This antibody is known to bind with the target antigen if it is present in the sample.
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If the target antigen is present in the sample, the fluorescent antibody will bind to it, forming an antigen-antibody complex.
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This complex can then be detected under a fluorescence microscope due to the fluorescent dye on the antibody. The presence and amount of fluorescence indicates the presence and quantity of the target antigen in the sample.
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Therefore, the Direct immunofluorescence test uses a known fluorescent antibody to detect the presence of a target antigen.
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