Which of the following is labeled with an enzyme in an ELISA test?Multiple choice question.Unknown antigenUnknown antibodyKnown antigenIndicator antibody of known specificity
Question
Which of the following is labeled with an enzyme in an ELISA test?Multiple choice question.Unknown antigenUnknown antibodyKnown antigenIndicator antibody of known specificity
Solution
In an ELISA (Enzyme-Linked Immunosorbent Assay) test, the component that is typically labeled with an enzyme is the "Indicator antibody of known specificity".
Here's a step-by-step explanation:
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The first step in an ELISA test is to attach the known antigen to the bottom of a well plate.
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The unknown antibody (which may or may not react to the antigen) is then added to the well. If the antibody recognizes the antigen, it will bind to it.
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The 'Indicator antibody of known specificity' is then added. This antibody is designed to attach to the unknown antibody, and it is labeled with an enzyme.
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A substrate is then added to the well. If the enzyme is present (meaning the indicator antibody has attached to the unknown antibody), the enzyme will cause a color change in the substrate. This color change indicates a positive result, meaning the unknown antibody does recognize the known antigen.
So, the correct answer to your question is "Indicator antibody of known specificity".
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