In ELISA, which of the following enzymes are conjugated to antibodies fordetection of the analyte?P. Alkaline phosphataseQ. TrypsinaseR. Horseradish peroxidaseS. Amylase
Question
In ELISA, which of the following enzymes are conjugated to antibodies fordetection of the analyte?P. Alkaline phosphataseQ. TrypsinaseR. Horseradish peroxidaseS. Amylase
Solution
In the Enzyme-Linked Immunosorbent Assay (ELISA), the enzymes that are typically conjugated to antibodies for the detection of the analyte are:
P. Alkaline phosphatase R. Horseradish peroxidase
These enzymes are used because they can catalyze reactions that result in a color change, which can be measured to determine the presence and quantity of the analyte.
Trypsinase (Q) and Amylase (S) are not typically used in ELISA.
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