Which of the following uses a known fluorescent antibody and a known antigen to detect the presence of a targeted unknown antibody?Multiple choice question.Direct immunofluorescence testingSandwich ELISAIndirect ELISARadioimmunoassayIndirect immunofluorescence testing
Question
Which of the following uses a known fluorescent antibody and a known antigen to detect the presence of a targeted unknown antibody?Multiple choice question.Direct immunofluorescence testingSandwich ELISAIndirect ELISARadioimmunoassayIndirect immunofluorescence testing
Solution
The correct answer is Indirect immunofluorescence testing.
Here's a step-by-step explanation:
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In indirect immunofluorescence testing, a known antigen is first introduced to the sample. This antigen is designed to bind to the targeted unknown antibody if it is present in the sample.
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After the antigen and sample have been incubated together, a fluorescent antibody is added. This antibody is known to bind to the antigen used in the first step.
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If the unknown antibody was present in the sample, the antigen will have bound to it. Then, the fluorescent antibody will bind to the antigen, creating a visible marker that indicates the presence of the unknown antibody.
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The sample is then examined under a microscope. If fluorescence is observed, it indicates that the unknown antibody is present in the sample. If no fluorescence is observed, it indicates that the unknown antibody is not present in the sample.
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