For successful multiple labelling immunohistochemistry (labelling two or more antigens in the same cell) which of the following is true?Group of answer choicesthe secondary antibodies must be raised in the same species as the piece of tissue being studiedthe fluorescent labels must share the same excitation wavelength (ie: be activated by the same fluorescent light colour)the primary antibodies must be raised in the same species as the piece of tissue being studiedthe secondary antibodies must be raised in different speciesthe primary antibodies must be raised in different species
Question
For successful multiple labelling immunohistochemistry (labelling two or more antigens in the same cell) which of the following is true?Group of answer choicesthe secondary antibodies must be raised in the same species as the piece of tissue being studiedthe fluorescent labels must share the same excitation wavelength (ie: be activated by the same fluorescent light colour)the primary antibodies must be raised in the same species as the piece of tissue being studiedthe secondary antibodies must be raised in different speciesthe primary antibodies must be raised in different species
Solution
The correct answer is "the secondary antibodies must be raised in different species".
Here's why:
In multiple labelling immunohistochemistry, you're trying to label two or more antigens (proteins) in the same cell. To do this, you use primary antibodies that bind to the specific antigens you're interested in. These primary antibodies are usually made in different species (like rabbit or mouse), so they have different "shapes" and can bind to different antigens.
Then, you use secondary antibodies that recognize and bind to these primary antibodies. The secondary antibodies are tagged with fluorescent labels, which allows you to see where the primary antibodies (and therefore the antigens) are located in the cell.
If the secondary antibodies were raised in the same species, they would all have the same "shape" and wouldn't be able to distinguish between the different primary antibodies. This would mean you couldn't
Similar Questions
Which of the following is labeled with an enzyme in an ELISA test?Multiple choice question.Unknown antigenUnknown antibodyKnown antigenIndicator antibody of known specificity
Which of the following uses a known fluorescent antibody and a known antigen to detect the presence of a targeted unknown antibody?Multiple choice question.Direct immunofluorescence testingSandwich ELISAIndirect ELISARadioimmunoassayIndirect immunofluorescence testing
Secondary antibodies are usually polyclonal because ________.more of them can bind to a primary antibodythey are more likely not to cross-react with target antigensthey are more specific to recognise an antibodythey can carry more reporter enzymes
The most unique and significant feature of monoclonal antibodies is that they are Blank______.Multiple choice question.composed of proteinproduced by plasma cells derived from the same B cellalways of the IgA classproduced by T cells derived from the same memory cell
Multiple Choice QuestionWhich of these is a lymphocyte that produces antibodies?Multiple choice question.NK cellB cellT cellMacrophage
Upgrade your grade with Knowee
Get personalized homework help. Review tough concepts in more detail, or go deeper into your topic by exploring other relevant questions.