How are antibody-antigen reactions visualized in an indirect ELISA test?Multiple choice question.Changes in amount of radioactivity in sampleBands appearing on blotter paperColor change of substrate converted to product by an enzymeObservations with a fluorescent microscope
Question
How are antibody-antigen reactions visualized in an indirect ELISA test?Multiple choice question.Changes in amount of radioactivity in sampleBands appearing on blotter paperColor change of substrate converted to product by an enzymeObservations with a fluorescent microscope
Solution
The visualization of antibody-antigen reactions in an indirect ELISA test is done through a color change of the substrate that is converted to a product by an enzyme. This is because, in an indirect ELISA, an enzyme-linked secondary antibody is used to react with a substrate to produce a detectable signal, typically a color change. Therefore, the correct answer is "Color change of substrate converted to product by an enzyme".
Similar Questions
Which of the following uses a known fluorescent antibody to detect the presence of a target antigen?Multiple choice question.Direct antigen testRadioimmunoassayIndirect immunofluorescence testDirect immunofluorescence testDirect ELISACapture ELISA
When an antigen-antibody reaction involves whole cells, the reaction can be observed Blank______.Multiple choice question.by detecting radioactive decayby looking for a color changewith a light microscope
In immunofluorescence testing, a fluorescent is attached to a specific antibody to provide a sensitive test for the presence of a specific antigen or antibody.
Which has improved the specificity of immunological testing systems?Multiple choice question.Use of radioactivityUse of monoclonal antibodiesUse of enzymesUse of electronic equipment
As opposed to the , the ELISA is a quantitative method.
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