How has it been proven that the DNA is the genetic material?A. The altering of an rRNA base did not change the protein’s primary structure.B. Killing R-type (not encapsulated) bacteria with heat and mixing them with S-type (encapsulated) bacteria lead to the death of mice injected with this mixture.C. Radioactive phosphorous isotope could be detected inside bacteria after an infection with bacteriophages containing this isotope, whereas radioactive sulphur isotope present in other, labelled phages could not.D. A and B
Question
How has it been proven that the DNA is the genetic material?A. The altering of an rRNA base did not change the protein’s primary structure.B. Killing R-type (not encapsulated) bacteria with heat and mixing them with S-type (encapsulated) bacteria lead to the death of mice injected with this mixture.C. Radioactive phosphorous isotope could be detected inside bacteria after an infection with bacteriophages containing this isotope, whereas radioactive sulphur isotope present in other, labelled phages could not.D. A and B
Solution
The proof that DNA is the genetic material comes from several key experiments:
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The Griffith's Experiment: This experiment involved two strains of Streptococcus pneumoniae bacteria - the R-type (rough and non-virulent) and the S-type (smooth and virulent). Griffith found that when he killed the S-type bacteria with heat and mixed them with live R-type bacteria, the R-type bacteria transformed into S-type bacteria and became virulent. This suggested that some "transforming principle" from the S-type bacteria was taken up by the R-type bacteria, changing their characteristics. This was later identified as DNA.
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The Avery-MacLeod-McCarty Experiment: This experiment followed up on Griffith's work. They treated the heat-killed S-type bacteria with enzymes that destroyed proteins, RNA, and other molecules, leaving only DNA. They found that the DNA could still transform R-type bacteria into S-type bacteria, suggesting that DNA was the "transforming principle".
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The Hershey-Chase Experiment: This experiment used bacteriophages (viruses that infect bacteria). They labeled the DNA of some phages with radioactive phosphorus and the protein of other phages with radioactive sulfur. After allowing the phages to infect bacteria, they found that only the radioactive phosphorus (from the DNA) was found inside the bacteria. This suggested that DNA, not protein, was the genetic material that was passed from the phages to the bacteria.
So, the correct answer to your question is C. Radioactive phosphorous isotope could be detected inside bacteria after an infection with bacteriophages containing this isotope, whereas radioactive sulphur isotope present in other, labelled phages could not.
Similar Questions
Hershey and Chase provided evidence that DNA was the genetic material in bacterial phage. In this landmark experiment, they used radiolabeled T2 bacteriophage to infect E. coli. They reasoned that since T2 could reprogram E. coli to produce only T2 phage particles, then the molecule that entered E. coli during an infection must be the genetic material. In this experiment:Group of answer choicesT2 protein was labeled in 32P since phosphorous atoms are present in several nonpolar amino acid.T2 protein was labeled with 35S since sulfur atoms are abundant in the amino groups of amino acids.T2 DNA was labeled with 32P since phosphorous atoms are found in the backbone of DNA.T2 DNA was labeled with 35S since sulfur atom are found in the nitrogenous bases.
Which of flowing virus was used by Hershey and Chase to prove that DNA is the chemical basis of heredity?*1 pointLambda phageColiphageT2 BacteriophageT4 Bacteriophage
35) En reacción a la replicación del DNA, indicar las frases incorrectas a) La replicación del cromosoma E.Coli la realiza exclusivamente la DNA-polimerasa I b) La obtención de los fragmentos de Okazaki en procariotas requiere de las actividades primasa y DNA pol III c) La DNA-pol I elimina los cebadores de RNA necesarios para el inicio de la extensión de l cadenas de DNA para la DNA polimerasa d) Durante la replicación las topoisomerasas no tienen un papel relevante e) La DNA polimerasa III de E.Coli no puede iniciar la replicación sin un cebador
how would you prove the bidirectional replication of DNA using radioisotopes?
Deoxyribonucleic acid (DNA) differs from ribonucleic acid (RNA) in that it:Group of answer choiceswas studied during Darwin’s lifetime.is the so-called recipe for all biological characteristics and functions in animals.was discovered by Mendel.is stored in ribosomes.
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