Hershey and Chase provided evidence that DNA was the genetic material in bacterial phage. In this landmark experiment, they used radiolabeled T2 bacteriophage to infect E. coli. They reasoned that since T2 could reprogram E. coli to produce only T2 phage particles, then the molecule that entered E. coli during an infection must be the genetic material. In this experiment:Group of answer choicesT2 protein was labeled in 32P since phosphorous atoms are present in several nonpolar amino acid.T2 protein was labeled with 35S since sulfur atoms are abundant in the amino groups of amino acids.T2 DNA was labeled with 32P since phosphorous atoms are found in the backbone of DNA.T2 DNA was labeled with 35S since sulfur atom are found in the nitrogenous bases.
Question
Hershey and Chase provided evidence that DNA was the genetic material in bacterial phage. In this landmark experiment, they used radiolabeled T2 bacteriophage to infect E. coli. They reasoned that since T2 could reprogram E. coli to produce only T2 phage particles, then the molecule that entered E. coli during an infection must be the genetic material. In this experiment:Group of answer choicesT2 protein was labeled in 32P since phosphorous atoms are present in several nonpolar amino acid.T2 protein was labeled with 35S since sulfur atoms are abundant in the amino groups of amino acids.T2 DNA was labeled with 32P since phosphorous atoms are found in the backbone of DNA.T2 DNA was labeled with 35S since sulfur atom are found in the nitrogenous bases.
Solution
The correct answer is: T2 DNA was labeled with 32P since phosphorous atoms are found in the backbone of DNA.
Here's the step by step explanation:
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Hershey and Chase conducted an experiment to determine whether DNA or proteins are the genetic material that viruses inject into host cells. They used T2 bacteriophage, a virus that infects E. coli bacteria.
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They labeled the DNA of some T2 phages with radioactive phosphorus-32 (32P). DNA contains phosphorus in its backbone (the sugar-phosphate backbone), but proteins do not contain phosphorus. Therefore, any 32P found inside the bacteria must have come from the phage DNA.
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They also labeled the proteins of some T2 phages with radioactive sulfur-35 (35S). Proteins contain sulfur in some amino acids, while DNA does not contain sulfur. Therefore, any 35S found inside the bacteria must have come from the phage proteins.
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They allowed the radiolabeled phages to infect E. coli. After the infection process, they found that the 32P (from DNA) was inside the bacteria, while the 35S (from proteins) was not.
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This provided strong evidence that DNA, not protein, is the genetic material that viruses inject into host cells.
Similar Questions
Which of flowing virus was used by Hershey and Chase to prove that DNA is the chemical basis of heredity?*1 pointLambda phageColiphageT2 BacteriophageT4 Bacteriophage
How has it been proven that the DNA is the genetic material?A. The altering of an rRNA base did not change the protein’s primary structure.B. Killing R-type (not encapsulated) bacteria with heat and mixing them with S-type (encapsulated) bacteria lead to the death of mice injected with this mixture.C. Radioactive phosphorous isotope could be detected inside bacteria after an infection with bacteriophages containing this isotope, whereas radioactive sulphur isotope present in other, labelled phages could not.D. A and B
Stanley Miller's 1952 experiment resulted in the creation of ____________ from simple inorganic molecules. Group of answer choicesProtocellsBacteriaAmino acidsProtein
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