Which of flowing virus was used by Hershey and Chase to prove that DNA is the chemical basis of heredity?*1 pointLambda phageColiphageT2 BacteriophageT4 Bacteriophage
Question
Which of flowing virus was used by Hershey and Chase to prove that DNA is the chemical basis of heredity?*1 pointLambda phageColiphageT2 BacteriophageT4 Bacteriophage
Solution
Hershey and Chase used the T2 Bacteriophage to prove that DNA is the chemical basis of heredity.
Here are the steps they followed:
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They grew some T2 bacteriophage in a medium that contained radioactive phosphorus (P32) and some others in a medium that contained radioactive sulfur (S35).
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The bacteriophages grown in the presence of P32 had radioactive DNA, while those grown in the presence of S35 had radioactive protein coats.
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They then allowed the radioactive phages to infect E. coli bacteria.
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After allowing enough time for infection to occur, they agitated the mixtures in a blender, which removed the phage coats from the bacteria.
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They then centrifuged the mixtures. The heavier bacteria formed a pellet at the bottom, while the lighter phage particles stayed in the supernatant (liquid above the pellet).
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They found that the P32 (which labels DNA) was in the bacterial pellet, while the S35 (which labels protein) was in the supernatant.
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This indicated that DNA, not protein, is the genetic material that is injected into bacteria during infection.
This experiment was a key piece of evidence showing that DNA is the molecule of heredity.
Similar Questions
Hershey and Chase provided evidence that DNA was the genetic material in bacterial phage. In this landmark experiment, they used radiolabeled T2 bacteriophage to infect E. coli. They reasoned that since T2 could reprogram E. coli to produce only T2 phage particles, then the molecule that entered E. coli during an infection must be the genetic material. In this experiment:Group of answer choicesT2 protein was labeled in 32P since phosphorous atoms are present in several nonpolar amino acid.T2 protein was labeled with 35S since sulfur atoms are abundant in the amino groups of amino acids.T2 DNA was labeled with 32P since phosphorous atoms are found in the backbone of DNA.T2 DNA was labeled with 35S since sulfur atom are found in the nitrogenous bases.
Which enzymes did Avery, MacLeod, and McCarty use in order to identify the genetic material?Multiple select question.Lipid digesting enzymesDNA digesting enzymesRNA digesting enzymesCarbohydrate digesting enzymes
How has it been proven that the DNA is the genetic material?A. The altering of an rRNA base did not change the protein’s primary structure.B. Killing R-type (not encapsulated) bacteria with heat and mixing them with S-type (encapsulated) bacteria lead to the death of mice injected with this mixture.C. Radioactive phosphorous isotope could be detected inside bacteria after an infection with bacteriophages containing this isotope, whereas radioactive sulphur isotope present in other, labelled phages could not.D. A and B
Which of the following is NOT associated with viruses?Group of answer choicesspikescapsidenvelopeorganellesnucleic acid
Which enzymes did Avery, MacLeod, and McCarty use in order to identify the genetic material?
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