In order to clone a sequence into a plasmid vector, the vector must contain certain sequences.What sequence is essential so that a plasmid vector will be maintained in the cells as they grow and divide?
Question
In order to clone a sequence into a plasmid vector, the vector must contain certain sequences.What sequence is essential so that a plasmid vector will be maintained in the cells as they grow and divide?
Solution
The sequence that is essential for a plasmid vector to be maintained in the cells as they grow and divide is called an "origin of replication" or "ori". This sequence is recognized by the replication machinery of the host cell, allowing the plasmid to be replicated each time the cell divides. Without an origin of replication, the plasmid would not be copied and would eventually be lost from the cell population.
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