Plasmids and viruses are common types ofMultiple choice question.proteins produced by transgenic organisms.cells that can be genetically-engineered to produce human proteins.STRs used in DNA profiling.cloning vectors.

To create a transgenic organism, researchers must use a cloning ______, such as a plasmid, to carry source DNA into a recipient cell.Multiple choice question.probevectorprimerendonuclease

Which of the following describes plasmids?Synthesise proteins.Controls what enters and exits the cell.Small circles of DNA that are used to share useful genes between bacteria.Contains all the instructions the bacterium needs to function and develop.

The first step in creating a transgenic organism is toMultiple choice question.insert recombinant DNA into recipient cells.synthesize or extract source DNA.isolate protein produced from recombinant DNA.cut a plasmid with restriction enzymes.

What is the most common vector used for genetic engineering in plants and where did it originally come from?What feature makes this plasmid useful in genetic engineering? [3]6. What are pharm animals? [2]7. Name two methods that can be used to introduce genetic material to create transgenic animals [2]8. Give three examples of transgenic animals discussed in lectures [3]9. What are the major concerns regarding genetically modified organisms as food? Give three. [3]10. What are biopharmaceuticals? Give 3 examples. [3]11. What is schmeat and what is the major limitation to this technology at the moment? [1]1. Name two feature of PCR that make this technology useful in molecular diagnostics and forensics [2]2. a) What are STRs and what makes them useful in forensic science?b) What is the major difference between RFLPs and STR analysis?3. In microarray technology:a) What must the mRNA be converted to before it may be bound by specific nucleotide probes and howis this done?b) How would you control the stringency of hybridization?c) Name two limitation of this technology4. In qPCR, what is the Ct value used to quantify gene expression?[3][1][2][2][2][2]

Which two applications of gene cloning involve transgenic organisms?