Which of the following vector(s) is(are) used to clone a DNA fragment of size220 kb?(A) Bacterial artificial chromosome(B) Yeast artificial chromosome(C) Cosmids(D) pUC19 plasmid
Question
Which of the following vector(s) is(are) used to clone a DNA fragment of size220 kb?(A) Bacterial artificial chromosome(B) Yeast artificial chromosome(C) Cosmids(D) pUC19 plasmid
Solution
The vectors that can be used to clone a DNA fragment of size 220 kb are:
(A) Bacterial artificial chromosome (BAC): This vector can carry a large DNA fragment of about 100-300 kb. Therefore, it can be used to clone a DNA fragment of size 220 kb.
(B) Yeast artificial chromosome (YAC): This vector can carry a very large DNA fragment of about 100-1000 kb. Therefore, it can also be used to clone a DNA fragment of size 220 kb.
(C) Cosmids: These vectors can carry a DNA fragment of about 35-45 kb. Therefore, they cannot be used to clone a DNA fragment of size 220 kb.
(D) pUC19 plasmid: This vector can carry a DNA fragment of about 2.5-5 kb. Therefore, it cannot be used to clone a DNA fragment of size 220 kb.
So, the vectors that can be used to clone a DNA fragment of size 220 kb are Bacterial artificial chromosome (BAC) and Yeast artificial chromosome (YAC).
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