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Human insulin is produced using

Question

Human insulin is produced using

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Solution

Human insulin is produced using a method known as recombinant DNA technology. Here are the steps involved:

  1. Isolation of Insulin Gene: The first step is to isolate the gene in the human body that produces insulin. This gene is responsible for the production of insulin in the body.

  2. Insertion of Insulin Gene into Plasmid: The isolated insulin gene is then inserted into a plasmid, which is a small, circular piece of DNA found in bacteria. This plasmid acts as a vehicle to carry the insulin gene into a bacterial cell.

  3. Transformation: The plasmid with the insulin gene is then introduced into a bacterial cell, usually E. coli. This process is known as transformation.

  4. Production of Insulin: Once inside the bacterial cell, the insulin gene starts producing insulin. The bacteria multiply and produce more insulin.

  5. Extraction and Purification: The insulin is then extracted from the bacteria and purified for use in humans.

This method of producing insulin is very efficient and has been used since the 1980s. It has greatly improved the availability of insulin for

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