Which reaction in DNA replication is catalysed by DNA ligase?Group of answer choicesBase pairing of the template to the newly synthesised strandFormation of a phosphodiester linkage between two Okazaki fragmentsProof reading the template strand for errorsAddition of nucleotides to the lagging strand
Question
Which reaction in DNA replication is catalysed by DNA ligase?Group of answer choicesBase pairing of the template to the newly synthesised strandFormation of a phosphodiester linkage between two Okazaki fragmentsProof reading the template strand for errorsAddition of nucleotides to the lagging strand
Solution
The reaction in DNA replication that is catalysed by DNA ligase is the formation of a phosphodiester linkage between two Okazaki fragments.
Here are the steps:
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During DNA replication, the DNA molecule unzips, and replication occurs in both directions, creating a replication fork.
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The leading strand is synthesized continuously in the direction of the replication fork.
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The lagging strand is synthesized discontinuously in the direction away from the replication fork. This results in the formation of Okazaki fragments.
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DNA ligase comes into play here. It catalyzes the formation of a phosphodiester linkage between the 3'-OH end of one fragment and the 5'-phosphate end of the next fragment.
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This action by DNA ligase effectively joins the Okazaki fragments together, creating a continuous DNA strand.
So, DNA ligase is crucial for the synthesis of the lagging strand during DNA replication.
Similar Questions
During DNA replication:Ligase replaces RNA primers with DNA nucleotides.DNA polymerase III pairs Guanine with Cytosine.DNA polymerase I pairs Adenine with Cytosine.Okazaki fragments are created on the leading strand.
Which is true during DNA replication?Group of answer choicesDNA ligase is the last step of the polymerisation reaction for the lagging strand, but is not used on the leading strand.DnaA binds to regions that are rich in AT as this purine-purine base pairing forms only two hydrogen bonds.The β sliding clamp prevents the dissociation of DNA polymerase III, increasing the length of DNA each polymerase can synthesise.DNA gyrase binds to and applies tension to the lagging strand.
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