Quantifying the concentration of protein using the Beer-Lambert Law is dependent on the presence of which ONE of the following?Group of answer choicesAromatic amino acidsDisulfide bondsThe ability to bind SDSPlanar peptide bonds
Question
Quantifying the concentration of protein using the Beer-Lambert Law is dependent on the presence of which ONE of the following?Group of answer choicesAromatic amino acidsDisulfide bondsThe ability to bind SDSPlanar peptide bonds
Solution
The Beer-Lambert Law is dependent on the presence of Aromatic amino acids. This is because the Beer-Lambert Law is used to measure the absorbance of light by a solution, and aromatic amino acids (like tryptophan, tyrosine, and phenylalanine) have conjugated systems that can absorb ultraviolet light. This absorbance can then be used to calculate the concentration of the protein.
Similar Questions
Ultraviolet (UV) light can be used to estimate protein solution concentrations because A. Alanine absorbs at 280 nm. B. all the amino acids absorb UV light. C. aliphatic amino acids absorb at 280 nm. D. tryptophan and tyrosine absorb at 280 nm. E. All of the above
Which of the following is least likely to cause denaturation of a protein?Group of answer choicesBoilingAdding a detergentPutting the protein in a more hypotonic solution
Beer-Lambert’s law gives a relation between
The Beer-Lambert Law is expressed as A=ϵbc. The symbol, c, refers toSelect one:capacitycohesioncatalystconcentration
Which type of reaction results in the formation of a peptide bond between amino acids and the loss of a water molecule?
Upgrade your grade with Knowee
Get personalized homework help. Review tough concepts in more detail, or go deeper into your topic by exploring other relevant questions.