Plasmids can be useful for many different elements of molecular microbiology but there are many considerations that dictate the features of a plasmid being used. If using a plasmid in order to express a large quantity of a particular protein (e.g. for protein purification) which of the following is NOT a requirement?The ability of the host bacterium to express GFP.A selectable marker that will facilitate retention of the plasmid.The presence of rep/ori genes that allows for replication of the plasmid in the bacterium of interest.A strong promoter that will drive transcription of the gene of interest and subsequent protein production.

Week 6 Moodle LessonMIC3011 S1 2024Week 6 Moodle LessonCompletion requirements DONE: VIEW DONE: GO THROUGH THE ACTIVITY TO THE ENDThis Moodle lesson will take you through the lecture content for Week 6.Plasmids can be useful for many different elements of molecular microbiology but there are many considerations that dictate the features of a plasmid being used. If using a plasmid in order to express a large quantity of a particular protein (e.g. for protein purification) which of the following is NOT a requirement?The ability of the host bacterium to express GFP.A selectable marker that will facilitate retention of the plasmid.The presence of rep/ori genes that allows for replication of the plasmid in the bacterium of interest.A strong promoter that will drive transcription of the gene of interest and subsequent protein production.

Discuss the role of plasmid in working of bacteria.

What considerations should be made for choosing a plasmid for protein expression experiments?Question 22Answera.Ensuring incompatible plasmids are present.b.No selectable markers for markerless insertion.c.The gene of interest’s native promoter.d.High copy number for increased protein production.

What is the role of the selectable marker in a plasmid for cloning in E coli? Group of answer choicesSo that the inserted gene can be expressedSo that the plasmid is maintained in the E coli cells as they grow and divide.So that only the E coli cells that take up the plasmid will survive.So a restriction enzyme can cut the plasmid in a specific location

Which of the following describes plasmids?Synthesise proteins.Controls what enters and exits the cell.Small circles of DNA that are used to share useful genes between bacteria.Contains all the instructions the bacterium needs to function and develop.