What is the role of the selectable marker in a plasmid for cloning in E coli? Group of answer choicesSo that the inserted gene can be expressedSo that the plasmid is maintained in the E coli cells as they grow and divide.So that only the E coli cells that take up the plasmid will survive.So a restriction enzyme can cut the plasmid in a specific location
Question
What is the role of the selectable marker in a plasmid for cloning in E coli? Group of answer choicesSo that the inserted gene can be expressedSo that the plasmid is maintained in the E coli cells as they grow and divide.So that only the E coli cells that take up the plasmid will survive.So a restriction enzyme can cut the plasmid in a specific location
Solution
The role of the selectable marker in a plasmid for cloning in E. coli is so that only the E. coli cells that take up the plasmid will survive.
Here's a step-by-step explanation:
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The selectable marker is a gene present on the plasmid, which confers resistance to an antibiotic (like ampicillin or kanamycin) to the bacteria.
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After the plasmid is introduced into the E. coli cells, the cells are grown on a medium containing the antibiotic.
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Only the cells that have taken up the plasmid (and thus, the resistance gene) will be able to survive in the presence of the antibiotic. The cells that did not take up the plasmid will be killed by the antibiotic.
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This allows for the selection of only those E. coli cells that have been successfully transformed with the plasmid.
So, the selectable marker does not directly help in the expression of the inserted gene, maintaining the plasmid in the E. coli cells, or in the cutting of the plasmid by a restriction enzyme. Its main role is in the selection of successfully transformed cells.
Similar Questions
What is the role of a polylinker or multi-cloning site in a plasmid used for cloning in E coli?Group of answer choicesSo that the inserted gene can be expressedSo a restriction enzyme can cut the plasmid in a specific locationSo that only the E coli cells that take up the plasmid will survive.So that the plasmid is maintained in the E coli cells as they grow and divide.
Plasmids can be useful for many different elements of molecular microbiology but there are many considerations that dictate the features of a plasmid being used. If using a plasmid in order to express a large quantity of a particular protein (e.g. for protein purification) which of the following is NOT a requirement?The ability of the host bacterium to express GFP.A selectable marker that will facilitate retention of the plasmid.The presence of rep/ori genes that allows for replication of the plasmid in the bacterium of interest.A strong promoter that will drive transcription of the gene of interest and subsequent protein production.
What considerations should be made for choosing a plasmid for protein expression experiments?Question 22Answera.Ensuring incompatible plasmids are present.b.No selectable markers for markerless insertion.c.The gene of interest’s native promoter.d.High copy number for increased protein production.
Which of the following describes plasmids?Synthesise proteins.Controls what enters and exits the cell.Small circles of DNA that are used to share useful genes between bacteria.Contains all the instructions the bacterium needs to function and develop.
State the role that the following factors play in gene cloning:Restriction enzymeLigasePlasmidVector
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