Week 6 Moodle LessonMIC3011 S1 2024Week 6 Moodle LessonCompletion requirements DONE: VIEW DONE: GO THROUGH THE ACTIVITY TO THE ENDThis Moodle lesson will take you through the lecture content for Week 6.Plasmids can be useful for many different elements of molecular microbiology but there are many considerations that dictate the features of a plasmid being used. If using a plasmid in order to express a large quantity of a particular protein (e.g. for protein purification) which of the following is NOT a requirement?The ability of the host bacterium to express GFP.A selectable marker that will facilitate retention of the plasmid.The presence of rep/ori genes that allows for replication of the plasmid in the bacterium of interest.A strong promoter that will drive transcription of the gene of interest and subsequent protein production.
Question
Week 6 Moodle LessonMIC3011 S1 2024Week 6 Moodle LessonCompletion requirements DONE: VIEW DONE: GO THROUGH THE ACTIVITY TO THE ENDThis Moodle lesson will take you through the lecture content for Week 6.Plasmids can be useful for many different elements of molecular microbiology but there are many considerations that dictate the features of a plasmid being used. If using a plasmid in order to express a large quantity of a particular protein (e.g. for protein purification) which of the following is NOT a requirement?The ability of the host bacterium to express GFP.A selectable marker that will facilitate retention of the plasmid.The presence of rep/ori genes that allows for replication of the plasmid in the bacterium of interest.A strong promoter that will drive transcription of the gene of interest and subsequent protein production.
Solution
The ability of the host bacterium to express GFP is NOT a requirement when using a plasmid to express a large quantity of a particular protein for protein purification. The other options listed - a selectable marker that will facilitate retention of the plasmid, the presence of rep/ori genes that allows for replication of the plasmid in the bacterium of interest, and a strong promoter that will drive transcription of the gene of interest and subsequent protein production - are all necessary features of the plasmid for this purpose. GFP, or Green Fluorescent Protein, is often used as a marker to visualize and track the activity of proteins in cells, but it is not necessary for the production or purification of proteins.
Similar Questions
Plasmids can be useful for many different elements of molecular microbiology but there are many considerations that dictate the features of a plasmid being used. If using a plasmid in order to express a large quantity of a particular protein (e.g. for protein purification) which of the following is NOT a requirement?The ability of the host bacterium to express GFP.A selectable marker that will facilitate retention of the plasmid.The presence of rep/ori genes that allows for replication of the plasmid in the bacterium of interest.A strong promoter that will drive transcription of the gene of interest and subsequent protein production.
Discuss the role of plasmid in working of bacteria.
Select the option that completes the sentence below.Plasmids……control what enters and exits the cell.…are small circles of DNA that are used to share useful genes between bacteria.…contain all the instructions the bacterium needs to function and develop.…synthesise proteins.
What considerations should be made for choosing a plasmid for protein expression experiments?Question 22Answera.Ensuring incompatible plasmids are present.b.No selectable markers for markerless insertion.c.The gene of interest’s native promoter.d.High copy number for increased protein production.
Which plasmid genes determine host specificity of a plasmid?Question 24Answera.Recombinases and repb.rep and oric.ori and partitioning genesd.Partitioning genes and recombinases
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