You are supplied with a 10mg/mL bovine serum albumin (BSA) stock solution made up in phosphate buffered saline (PBS). You need to make a 2mg/mL BSA standard solution from the BSA stock solution. How much 10mg/mL BSA stock solution and how much phosphate buffered saline (PBS) do you need to make 10mL of a 2mg/mL BSA working standard.
Question
You are supplied with a 10mg/mL bovine serum albumin (BSA) stock solution made up in phosphate buffered saline (PBS). You need to make a 2mg/mL BSA standard solution from the BSA stock solution. How much 10mg/mL BSA stock solution and how much phosphate buffered saline (PBS) do you need to make 10mL of a 2mg/mL BSA working standard.
Solution
To prepare a 2mg/mL BSA solution from a 10mg/mL BSA stock solution, you need to use the formula C1V1 = C2V2, where C1 is the initial concentration, V1 is the initial volume, C2 is the final concentration, and V2 is the final volume.
Here, you want to find out V1, the volume of the 10mg/mL BSA stock solution you need. So, rearrange the formula to solve for V1:
V1 = (C2V2) / C1
Substitute the given values into the formula:
V1 = (2mg/mL * 10mL) / 10mg/mL
V1 = 2mL
So, you need 2mL of the 10mg/mL BSA stock solution.
To find out how much PBS you need, subtract the volume of the BSA solution you're using from the total volume of the solution you want to make:
PBS volume = Total volume - BSA volume
PBS volume = 10mL - 2mL
PBS volume = 8mL
So, to make 10mL of a 2mg/mL BSA solution, you need 2mL of a 10mg/mL BSA stock solution and 8mL of PBS.
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