Knowee
Questions
Features
Study Tools

You are supplied with a 10mg/mL bovine serum albumin (BSA) stock solution made up in phosphate buffered saline (PBS). You need to make a 2mg/mL BSA standard solution from the BSA stock solution. How much 10mg/mL BSA stock solution and how much phosphate buffered saline (PBS) do you need to make 10mL of a 2mg/mL BSA working standard.

Question

You are supplied with a 10mg/mL bovine serum albumin (BSA) stock solution made up in phosphate buffered saline (PBS). You need to make a 2mg/mL BSA standard solution from the BSA stock solution. How much 10mg/mL BSA stock solution and how much phosphate buffered saline (PBS) do you need to make 10mL of a 2mg/mL BSA working standard.

🧐 Not the exact question you are looking for?Go ask a question

Solution

To prepare a 2mg/mL BSA solution from a 10mg/mL BSA stock solution, you need to use the formula C1V1 = C2V2, where C1 is the initial concentration, V1 is the initial volume, C2 is the final concentration, and V2 is the final volume.

Here, you want to find out V1, the volume of the 10mg/mL BSA stock solution you need. So, rearrange the formula to solve for V1:

V1 = (C2V2) / C1

Substitute the given values into the formula:

V1 = (2mg/mL * 10mL) / 10mg/mL

V1 = 2mL

So, you need 2mL of the 10mg/mL BSA stock solution.

To find out how much PBS you need, subtract the volume of the BSA solution you're using from the total volume of the solution you want to make:

PBS volume = Total volume - BSA volume

PBS volume = 10mL - 2mL

PBS volume = 8mL

So, to make 10mL of a 2mg/mL BSA solution, you need 2mL of a 10mg/mL BSA stock solution and 8mL of PBS.

This problem has been solved

Similar Questions

You have a solution containing 10mg/ml of protein bovine serum albumin (BSA). MW of BSA is 68000. Calculate the concentration of this solution expressed as mM (Express your answer to two decimal places, don't forget the unit symbol, do not place a space between the numerical value and the unit symbol in your answer).

To construct a BSA standard curve, a dilution series needs to be made using the 2mg/ml BSA standard prepared above. Use the formula C1V1 = C2V2 and determine the volumes of the 2mg/ml standard that you need to dilute in PBS to make 1mL or 1000ul of each BSA working standard at concentrations ranging from 0.1mg/ml to 1.5mg/ml BSA

Injections of gonadotropins (GnRH) are used to stimulate follicle development during in vitro fertilisation. A patient needs to be given a 2 mL injection containing 600 International Units (IU) of gonadotropin, but you are given a solution which contains 750 IU in 1 mL.Work out how much of this gonadotropin solution you will need to give the patient and then in the box below write how much 0.9% saline you need to add to this solution to make the injection volume up to 2 mL (Write your answer in mL with ONE decimal place and NO UNITS)ChatGPTChatGPT

The primary healthcare provider (PHCP) prescribes 250 mL of 0.9% saline to infuse over 75 minutes. How many mL per hour will be administered to the client? Fill in the blank.

The solution used in hospitals for intravenous infusion, called physiological saline or saline solution, is 0.155 M in NaCl. It is typically prepared by diluting a 1.63 M stock solution with water. What volume (L) of the 1.63 M stock solution is required to prepare 2.00 L of the 0.155 M saline solution?

1/2

Upgrade your grade with Knowee

Get personalized homework help. Review tough concepts in more detail, or go deeper into your topic by exploring other relevant questions.