After Gram's iodine is added, what color do the cells appear under a light microscope?
Question
After Gram's iodine is added, what color do the cells appear under a light microscope?
Solution
After Gram's iodine is added, the cells appear dark purple or blue under a light microscope. This is because the iodine forms a complex with the crystal violet that was previously added, trapping it in the cell. This is the coloration you would see in Gram-positive bacteria. If the bacteria are Gram-negative, they will lose this color after the decolorization step.
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