The Gram’s stain used for bacterial staining isSelect an answerAsafranin and iodineBcrystal violet and cotton blueCcotton blue and iodineDcrystal violet and iodine
Question
The Gram’s stain used for bacterial staining isSelect an answerAsafranin and iodineBcrystal violet and cotton blueCcotton blue and iodineDcrystal violet and iodine
Solution
The correct answer is D: crystal violet and iodine.
Gram's stain is a method of staining used in microbiology. It differentiates bacteria into two large groups: Gram-positive and Gram-negative. The method has four basic steps:
- Application of a primary stain (Crystal Violet)
- The addition of Gram's iodine
- Decolorization with alcohol or acetone
- Counterstain with safranin
In this process, crystal violet and iodine are used, making option D the correct answer.
Similar Questions
The second dye used in gram staining is ___. methylene bluecrystal violetiodinesafranin
The key differential step in the Gram stain is: Group of answer choices none is considered differential the application of crystal violet the application of the iodine mordant application of safranin red dye the alcohol wash step
You are performing a Gram stain on gram-positive bacteria and you stop after the addition of the first dye. What is the appearance of the bacteria at this point?Question 13Answera.purpleb.colorlessc.redd.brown
Gram staining is based on:a) the ability of the bacterial cell wall to retain crystal violet dyeb) the lysis of bacterial cellsc) differences in bacterial shape and sized) growth of bacterial cells
Gram staining involves three steps: 1. crystal violet (purple) staining, 2. decolorization, 3. counterstaining (red). If you look under a microscope after the first step, you will see that:Group of answer choicesonly gram negative bacteria are purpleonly gram positive bacteria are purpleboth gram positive and negative bacteria are purpleneither gram positive and negative bacteria are purple
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