What is the role of the selectable marker in a plasmid for cloning in E coli? Group of answer choicesSo a restriction enzyme can cut the plasmid in a specific locationSo that only the E coli cells that take up the plasmid will survive.So that the inserted gene can be expressedSo that the plasmid is maintained in the E coli cells as they grow and divide.

What is the role of a polylinker or multi-cloning site in a plasmid used for cloning in E coli?Group of answer choicesSo that the inserted gene can be expressedSo a restriction enzyme can cut the plasmid in a specific locationSo that only the E coli cells that take up the plasmid will survive.So that the plasmid is maintained in the E coli cells as they grow and divide.

Plasmids can be useful for many different elements of molecular microbiology but there are many considerations that dictate the features of a plasmid being used. If using a plasmid in order to express a large quantity of a particular protein (e.g. for protein purification) which of the following is NOT a requirement?The ability of the host bacterium to express GFP.A selectable marker that will facilitate retention of the plasmid.The presence of rep/ori genes that allows for replication of the plasmid in the bacterium of interest.A strong promoter that will drive transcription of the gene of interest and subsequent protein production.

What considerations should be made for choosing a plasmid for protein expression experiments?Question 22Answera.Ensuring incompatible plasmids are present.b.No selectable markers for markerless insertion.c.The gene of interest’s native promoter.d.High copy number for increased protein production.

Which of the following describes plasmids?Synthesise proteins.Controls what enters and exits the cell.Small circles of DNA that are used to share useful genes between bacteria.Contains all the instructions the bacterium needs to function and develop.

State the role that the following factors play in gene cloning:Restriction enzymeLigasePlasmidVector