You have DNA with a concentration of 100 ng/µl and you need to have 100 µl of a diluted DNA solution with a concentration of 25 ng/µl. How much DNA will you dilute? Give the volume of DNA stock and water in your dilution. A. 25 µl DNA + 100 µl water B. 4 µl DNA + 21 µl water C. 75 µl DNA + 25 µl water D. 25 µl DNA + 75 µl water
Question
You have DNA with a concentration of 100 ng/µl and you need to have 100 µl of a diluted DNA solution with a concentration of 25 ng/µl. How much DNA will you dilute? Give the volume of DNA stock and water in your dilution. A. 25 µl DNA + 100 µl water B. 4 µl DNA + 21 µl water C. 75 µl DNA + 25 µl water D. 25 µl DNA + 75 µl water
Solution
The answer is D. 25 µl DNA + 75 µl water.
Here's the step-by-step calculation:
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First, determine the total amount of DNA you need. This is the final volume times the final concentration: 100 µl * 25 ng/µl = 2500 ng.
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Then, determine how much of your stock solution you need to get that amount of DNA. This is the total
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