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Which of the following is a 1:4 dilution?Group of answer choices15 uL PBS + 5 uL antibody16 uL PBS + 4 uL antibody20 uL PBS + 5 uL antibody20 uL PBS + 4 uL antibody

Question

Which of the following is a 1:4 dilution?Group of answer choices15 uL PBS + 5 uL antibody16 uL PBS + 4 uL antibody20 uL PBS + 5 uL antibody20 uL PBS + 4 uL antibody

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Solution

The 1:4 dilution from the given options is 16 uL PBS + 4 uL antibody.

Here's the step by step explanation:

  1. A 1:4 dilution means that the mixture contains 1 part of the substance of interest (in this case, the antibody) for every 4 parts of the diluent (in this case, PBS).

  2. To find the correct answer, you need to look for the option where the ratio of the volume of antibody to the total volume of the mixture (antibody + PBS) is 1/5 (which is equivalent to 1:4).

  3. If you calculate the ratios for each option, you

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